Fig. 5
Genes downregulated by miR-101-3p in HCT116. HCT116 cells were transfected with control miRNA (PMC) and miR-101-3p and submitted to qPCR (n = 3) or Western Blot (for 36 or 48 h) analysis. (a) Expression of potential direct and indirect miR-101-3p targets in miR-101-3p transfected HCT116 cells, relative to control (set as 1). Data is expressed as mean ± SD (n = 3). * p < 0.01, **p < 0.001, *** p < 0.0001; unpaired two-tailed t-test. (b) Western blot image showing band densitometry quantification of the long anti-apoptotic (MCL-1L) and short pro-apoptotic (MCL-1S) protein isoforms in HCT116 cells following transfection with miR-101-3p, relative to PMC-transfected cells (normalized for protein input, based on ß-actin bands). (c) MCL-1 protein interaction network. Red circles indicate genes downregulated by miR-101-3p in our microarray analysis