Fig. 6

Migration and invasion assays. Transwell migration a and invasion b assays are depicted. A graph with a quantitative analysis is shown for each pair of cells. Normal C57/Bl6 MSCs were used as negative controls, whereas the breast cancer cell line MDAMB231 was the positive control. All assays were done in triplicate. Statistical significance is marked with an asterisk “*”. a For non-irradiated cells in the migration assay, we found that the mean number of migratory cells per plate was 597 (95% CI 497–696) for Ctrl shRNA cells compared to 789 (95% CI 759–818) for Stag2 shRNA cells (p = .004). For radiated cells, mean number of migratory cells per plate was 640 (95% CI 538–742) for Ctrl shRNA+10Gy migratory cells per plate compared to 857 (95% CI 785–929) for Stag2 shRNA+10Gy cells (p = .002). b For non-irradiated cells in the invasion assay, the mean number of invasive cells per plate was 749 (95% CI 704–794) for Ctrl shRNA compared to 914 (95% CI 831–996) for Stag2 shRNA cells (p = .006). For the radiated cells, the mean number of invasive cells per plate was 542 (95% CI 4907–594) for Ctrl shRNA+10Gy compared to 676 (95% CI 601–751) for Stag2 shRNA+10Gy cells (p = .008)