Fig. 4
The G-quadruplex stabilizer GQC-05 is synergistic with Navitoclax in AML cell lines. The AML cell lines were treated with GQC-05 ranging from 20 to 280 nM for KG-1a and 100–600 nM for CMK and TF-1, in combination with Navitoclax ranging from 2 nM – 2 μM. Cells were assayed for cell number after 72 h of treatment and normalized to untreated cells. a Dose response curves for Navitoclax for each cell line in combination with no GQC-05, or approximate EC50 or EC25 doses. Doses in the shaded area were analyzed for synergy using Chou-Talalay analysis. b Chou-Talalay plots for each cell line treated with the combination of GQC-05 and Navitoclax where the Log10 of combination index (CI) is plotted against the fraction inhibited (Fa). Log10 CI < − 0.10 is synergistic; Log10 (CI) ≥ − 0.10 and < 0.08 is additive, and Log10 CI ≥ 0.08 is antagonistic