Fig. 4

Effects of Numbl plasmids on the expression of Numbl. a The HA-Numbl plasmid, empty plasmid (a) and RNAi plasmids (b) or a control RNAi plasmid were transfected into RPMI 8226 and H929 cells for 48 h. Expression levels of Numbl were assessed by Western blot analysis. c The mutant N7 (lacking PTB domain) and N8 (lacking c-terminal domain) were transfected respectively