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Fig. 3 | BMC Cancer

Fig. 3

From: Elucidating the expression and function of Numbl during cell adhesion-mediated drug resistance (CAM-DR) in multiple myeloma (MM)

Fig. 3

Identification of domains required for the interaction between Numbl and Integrin β1. a A schematic presentation of designed human Numbl derivatives. Numbl contains a phosphotyrosine binding region (PTB), a coiled-coil domain (CC), and a Phe-rich segment. b Schematic diagram of Integrin β1 gene and domain. c Two regions of Numbl are involved in its interaction with Integrin β1. HEK293T cells were co-transfected with GFP-Integrin β1 and HA-Numbl derivatives. Cell lysates were immunoprecipitated with anti-HA antibody and analyzed by Western blots with anti-GFP antibody. d A short N-terminal fragment (amino acids: 455–802) is required for binding with Integrin β1. HEK293T cells were transfected with the indicated expression plasmids. Immunoprecipitation and Western Blot analysis were performed using indicated antibodies

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