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Fig. 1 | BMC Cancer

Fig. 1

From: ROS as a novel indicator to predict anticancer drug efficacy

Fig. 1

Correlation between the baseline ROS level, mtDNA copy number and drug sensitivity. Intracellular ROS (a) and mitochondrial superoxide (b) levels of the Ishikawa, MDA-MB-231, PC-3 and Caco-2 cell lines are represented by the fluorescence intensity of H2DCFDA and MitoSOX respectively. (c) Relative content of mtDNA in Ishikawa, MDA-MB-231, PC-3 and Caco-2 normalised against the house keeping gene (β-actin). The columns represent the relative mtDNA copy numbers of the cell lines. Comparison of the CDDP IC50s (d) and the DQA IC50s (e) amongst the 4 cell lines. The columns represent the CDDP IC50s and the DQA IC50s in the cell lines. Data are mean ± SEM (N = 3 separate experiments) in all of the figure panels; p values were calculated using one-way ANOVA with Tukey multiple comparison post-hoc analysis; *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001

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