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Fig. 5 | BMC Cancer

Fig. 5

From: TIMP-2 secreted by monocyte-like cells is a potent suppressor of invadopodia formation in pancreatic cancer cells

Fig. 5

TIMP2-mediated inhibition of invadopodia is reversible after washout in pancreatic cancer cells. a Representative images from a PaTu8902 invadopodia assay where the cells were either incubated for 48 h with control medium, control medium with GM6001 (10 μM) washed out after 5 h and replaced with fresh control media or GM6001 (10 μM) left for 48 h. b Quantification of degradation per field of view for experimental conditions described above (a). c Representative images from a PaTu8902 invadopodia assay where the cells were either incubated for 48 h with control medium, control medium with recombinant TIMP2 (rTIMP2, 50 ng/ml) washed out after 5 h and replaced with fresh control medium or control medium with rTIMP2 (50 ng/ml) left for 48 h. d Quantification of degradation per field of view for experimental conditions described above (c). There was no significant difference in the amount of gelatin degradation between the control cells and the cells where rTIMP2 had been removed post 5 h incubation indicating that PaTu8902 cells were able to recover invadopodia activity once rTIMP2 had been removed. In contrast, again, those cells continuously exposed to rTIMP2 revealed significantly reduced invadopodia formation. e Representative images from a CFPAC-1 invadopodia assay where the cells were either incubated for 48 h with control medium, control medium with GM6001 (10 μM) washed out after 5 h and replaced with fresh control media or GM6001 (10 μM) left for 48 h. f Quantification of degradation per field of view for experimental conditions described above (e). g Representative images from a CFPAC-1 invadopodia assay where the cells were either incubated for 48 h with control medium, control medium with recombinant TIMP2 (rTIMP2, 50 ng/ml) washed out after 5 h and replaced with fresh control media or rTIMP2 (50 ng/ml) left for 48 h. h Quantification of degradation per field of view for experimental conditions described above. In all cases **** = p < 0.0001, *** = p < 0.001, ** = p < 0.01. Experiments were repeated three times

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