Fig. 4

TIMP2 inhibits invadopodia formation in PDAC cells. a Representative images from a PaTu8902 invadopodia assay where the cells were either incubated with THP1 conditioned medium (THP1-CM), or were cultured with recombinant TIMP1 (rTIMP1) or recombinant TIMP2 (rTIMP2) at indicated concentrations. b and c Quantification of degradation per field of view for experimental conditions described above (a). d Representative images from a CFPAC-1 invadopodia assay where the cells were either incubated with THP1 conditioned medium (THP1-CM), or were cultured with recombinant TIMP1 (rTIMP1) or recombinant TIMP2 (rTIMP2) at indicated concentrations. e and f) Quantification of degradation per field of view for experimental conditions described above (d). In all cases **** = p < 0.0001, ** = p < 0.01, *** = p < 0.001. Experiments were repeated three times