Fig. 3

THP1 cells secrete TIMP1 and TIMP2. a Representative images from a PaTu8902 invadopodia assay where the cells were either incubated with control medium, conditioned medium that had been applied fresh, boiled or freeze/thawed as indicated. b Quantification of degradation per field of view for experimental conditions described above (a). c Representative images from a CFPAC-1 invadopodia assay where the cells were either incubated with control medium, conditioned medium that had been applied fresh, boiled or freeze/thawed as indicated. d Quantification of degradation per field of view for experimental conditions described above (c). e THP1 conditioned media protein micro array (see methods for details). f concentrated samples of THP1 CM and RPMI 1640 control were immunoblotted for TIMP1 using specific antibodies. g control media containing recombinant TIMP1 was immunoblotted in comparison to aliquots of concentrated samples of THP1 CM for TIMP1 using specific antibodies. Molecular weight markers indicated for TIMP 1 (19 kD) and TIMP 2 (24 kD). h concentrated samples of THP1 CM and RPMI 1640 control were immunoblotted for TIMP2 using specific antibodies. Molecular weight markers indicated for TIMP 1 (19 kD) and TIMP 2 (24 kD). i control media containing recombinant TIMP1 was immunoblotted in comparison to a concentrated sample of THP1 CM using TIMP2 using specific antibodies. Molecular weight markers indicated for TIMP 1 (19 kD) and TIMP 2 (24 kD). Experiments were repeated three times. j control media containing recombinant TIMP2 was immunoblotted in comparison to a concentrated sample of THP1 CM using TIMP2 using specific antibodies. Note, double bands in Fig. 3j are considered dimers of the TIMP2 molecule. All experiments were repeated three times. Molecular weight markers indicated for TIMP 1 (19 kD) and TIMP 2 (24 kD)