Fig. 1

PaTu8902 and CFPAC-1 cells exhibit robust invadopodia activity. a Pancreatic ductal adenocarcinoma (PDAC) cell lines were analysed for seeded on fluorescent gelatin for 24 h fixed and stained for cortactin and DAPI. Cortactin puncta corresponding with black dots on the gelatin were considered active invadopodia. b Quantification of degradation per field of view for (a), MDA-MB-231 cells were also seeded on fluorescent gelatin for 24 h and the degradation per field of view calculated as a positive control. c PaTu8902, CFPAC-1 and PaTu8988-S cell lysates were blotted for MT1-MMP. PaTu8902 and CFPAC-1 show high levels of MT1-MMP expression whereas PaTu8988-S shows no MT1-MMP expression. d Western blot of PaTu8902 and CFPAC-1 cell lysates showing high levels of MMP9 expression. e Western blot of PaTu8902 and CFPAC-1: indicating no expression of TIMP2