Fig. 2From: Human papillomavirus type 18 oncoproteins exert their oncogenicity in esophageal and tongue squamous cell carcinoma cell lines distinctlyEffects of downregulation of HPV18 E6 and E7 on p53, pRB and its related pocket proteins in EC109, EC9706 and Tca83 cells. These cells were transfected with siRNA against control (−) or against HPV18 E6 and E7 (+). After 72 h, total lysate was collected and the levels of E6 and E7 target proteins were analyzed by Western blotting for the proteins as indicated. HeLa and HKESC01 cells were included as positive and negative controls, respectively. a. Representative immunoblots showing levels of E7 (pRB, p103 and p107) (navy blue-colored bars) and E6 (p53, p21 and hDlg) (light blue-colored bars) target proteins. β-actin was included as a loading control. b. Bar graphs show quantitation of the levels of target proteins against control (siCtrl) in (i) HeLa, (ii) EC109, (iii) EC9706, (iv) Tca83 and (v) HKESC01 cells. Quantitation was performed using ImageJ software and statistical analysis was performed using Prism. Error bars represent mean ± standard deviation (SD) (n = 4). (*P < 0.05, **P < 0.01, ***P < 0.001)Back to article page