Fig. 3

In vivo metastasis formation and tumor cell dissemination of HEYL-overexpressing cells after sub-renal capsular xenotransplantation. a HEYL mRNA expression in kidney capsule xenograft tumors after transduction of cells with lentiviral overexpression vectors and xenotransplantation into mice. Relative expression levels were quantified via qRT-PCR as fold-expression calibrated to HEK-293 T control with GAPDH as internal control. Data for one representative primary tumor is shown per condition. Depicted are mean and standard deviation (n = 3). b-c Metastasis formation of M1 (b) and NM1 (c) cells after lentiviral HEYL overexpression compared to control. Shown as a bar chart is the proportion of mice with and without any distant metastases to solid organs (left), as a scatterplot the total number of liver (and lung) metastases with labelling of metastases at other organ sites (middle) and as bar chart the proportion of mice with tumor dissemination into the bone marrow (right). Total numbers of mice (n) within the bar charts are depicted on the right side of the bar. Median and interquartile range are shown within the scatterplot. Significant differences are marked with an asterisk (*, p < 0.05; **, p < 0.01). MET, metastases; TCD_BM, tumor cell dissemination into the bone marrow; NST, nuchal stroma metastasis