Fig. 5

SDC-1 inhibited the phosphorylation of Ras/Raf/MEK/ERK pathway. pcDNA3.1 or pc-SDC-1 was transfected into SW620 and SW480 cell lines. a-b Western blot analysis was utilized to evaluate the protein levels of Ras, Raf, p-MEK and p-ERK. β-actin was used as an internal reference for normalizing the protein expression. ***p < 0.001