Fig. 3
From: Detection of carcinogen-induced bladder cancer by fluorocoxib A
Progression of bladder cancer by BBN. The BBN-induced bladder cancer progression from normal urothelium to hyperplasia/CIS and to invasive carcinoma with increased inflammation in bladder of mice was confirmed by H & E staining (left panels). The progression of bladder carcinogenesis was confirmed by increased expression of Ki67 positive urothelial cells in bladder from mice exposed to BBN (middle panels). In addition, uroplakin-1A, a protein that is highly expressed in normal bladder urothelium was downregulated in BBN-induced bladder cancers as confirmed by IHC analysis (right panels). Images were taken by Leitz DMRB microscope, scale bar 100 μm. C – carcinoma; CIS – carcinoma in situ; H – hyperplasia; I - inflammation; U - normal urothelium