Table 1 Details of primary antibodies
Antibody | Laboratory | Immunofluorescence | Western blot |
|---|---|---|---|
Gankyrin | Novus | 1 in 10,000 | 1 in 1000 |
GAPDH | Abcam | N/A | 1:5000 |
MAGE-A4 | gift | 1:300 | N/A |
POU5F1 | Santa Cruz | 1:150 | 1:300 |
SOX9 | Merck Millipore | 1:5000 | N/A |
TP53 | Santa Cruz | 1:1000 | 1:1000 |
Tubulin | Abcam | N/A | 1:5000 |