Fig. 7

Effect of Gankyrin knock-down on cisplatin sensitivity in NTera2 cells. a Gankyrin mRNA expression after Gankyrin knock-down in cisplatin (20 nM) exposed NTera2 cells. b Gankyrin knock-down and cisplatin treatment effect on the percentage of surviving cells Gankyrin knock-down and cisplatin treatment effects on (c) TP53 mRNA and (d) protein expression. e Representative image for TP53 western blot in Vehicle (V) and Gankyrin siRNA transfected (T) samples with and without cisplatin treatment and a no treatment control (NT). f Relative FAS mRNA expression after Gankyrin knock-down and cisplatin treatment. CTL: control, CISP: cisplatin, VEH + CISP: vehicle and cisplatin, siRNA+CISP: Gankyrin siRNA+cisplatin. Data analysed by paired t-test, means ±SEM, **p < 0.001, *p < 0.05. Each data point represents the mean of an individual experiment, each with 3 replicates. Paired samples from an individual experiment are represented by the same colour.