Effect of Gankyrin knock-down on cell number, cell proliferation and cell cycle of NTera2 cells. a Gankyrin knock-down effect on NTera2 cell number. Relative PCNA (b) and ki67 (c) expression after 24 h of Gankyrin siRNA transfection. Distribution of NTera2 cells in GO/G1 phase (d), G2/M phase (e) or S phase (f). Data analysed by paired t-test, means ± SEM, ** p < 0.001, *p < 0.05. Each data point represents the mean of an individual experiment, each with an 3 replicates. Paired samples from an individual experiment are represented by the same colour. (g) Representative image of the flow cytometry plot obtained during cell cycle experiments.