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Fig. 2 | BMC Cancer

Fig. 2

From: LINC01128 expedites cervical cancer progression by regulating miR-383-5p/SFN axis

Fig. 2

SFN functions as a target gene of miR-383-5p. a. The interference efficiency of miR-107 and miR-383-5p were estimated by RT-qPCR in HeLa and SiHa cells. b. The expression of SFN was determined via RT-qPCR after overexpressing miR-107 and miR-383-5p. c. The protein level of SFN was detected by western blot. d. miR-383-5p expression in CC tissues and adjacent normal tissues was measured by RT-qPCR. e. RNA pull down assay examined the binding association of LINC01128 and miR-383-5p by which precipitated with miR-383-5p no-biotin probe or miR-383-5p biotin probe. f. The predicting binding sites between LINC01128 and miR-383-5p were obtained from starBase website. g. Luciferase reporter assay was implemented to test the interaction relationship between LINC01128 and miR-383-5p. h. HeLa and SiHa cells were transfecting with miR-383-5p-mimics. Cell proliferation ability was analyzed by CCK-8 assay. i. Bax and Bcl-2 protein levels were checked by western blot. **p < 0.01

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