Fig. 6

Effect of SOCS1 on 8305c and FRO cell proliferation and invasion. a 8305c and FRO cells were instantaneous transfected with SOCS1-overexpressing vector or negative control (8305c: 85.76 ± 5.22 vs. 42.33 ± 3.72 [control], P = 0.003; FRO: 93.41 ± 5.79 vs. 51.76 ± 3.21 [control], P < 0.001). b Cell survival rates in MTT assay in SOCS1 overexpression and control 8305c and FRO cells. The rate of increase in cell number decreased in SOCS1 overexpression cells was found (8305c: 23.6% ± 2.2, 31.1% ± 3.5, 38.7% ± 3.9 and 42.2% ± 5.7 at 24, 48, 72, and 96 h, respectively, P = 0.0013; FRO: 13.5% ± 1.5, 29.5% ± 3.1, 38.1% ± 4.6 and 40.8% ± 6.1 at 24, 48, 72, and 96 h, separately, P = 0.019). c Typical photo (upper; zoom in 200 times) and fixed quantity discuss (bottom) of determination of Transwell mobile and aggressive attacks in SOCS1 overexpression and negative dominate 8305c and FRO cells (8305c, P = 0.019; FRO: P = 0.036). All trials are done three times