Fig. 4

SOCS1 is a direct target of miR-155 in ATC. a Western special blot and number of SOCS1 protein expression in 8305c and FRO cells infection with miR-155 mimic or passive bridle (8305c: 81.45 ± 7.11 vs. 41.55 ± 3.17, P = 0.033; FRO: 90.33 ± 5.51 vs. 52.13 ± 2.98, P = 0.048). Using β-actin as a reference. b miR-155 diametrically interplay with the 3′-UTRs of SOCS1. c The relative luciferase activity of the reporter with natural growth-type SOCS1 3′-UTR was significantly decreased after transfection with miR-155 mimic. Nevertheless, after the mutation of 3′-UTR with SOCS1, the luciferase activity of the reporter was not affected by miR-155. The data obtained is the average ± SD. The log transformed data was analyzed using two-way analysis of variance. **P = 0.008 and P = 0.017 for 8305c and FRO cells, respectively