Fig. 6

Aurora-a promotes the activation of NF-κB signaling in HCC cells. a Western blotting was used to detect the nuclear or cytoplasmic IκBα protein expression in parental and their radioresistant HCC cells. The internal control was GAPDH or Topo I, respectively. b Western blotting detection of p65 protein expression in parental and their radioresistant HCC cells. The internal control was GAPDH. c A luciferase reporter system was used to measure the activity of NF-κB in parental and their radioresistant HCC cells. d Western blotting was used to detect the nuclear or cytoplasmic IκBα protein and p65 expression in the Aurora-A-knockdown HCC cells. The internal control was GAPDH or Topo I, respectively. e Western blotting was used to detect the nuclear or cytoplasmic IκBα protein and p65 expression in the Aurora-A-overexpressing HCC cells. The internal control was GAPDH or Topo I, respectively. f A luciferase reporter system was used to measure the activity of NF-κB in the stably transfected HCC cells. g and h Western blotting was used to detect the apoptosis-related proteins (Bcl-2, Mcl-1, cleaved PARP and caspase-3) in the Aurora-A-knockdown and Aurora-A-overexpressing HCC cells. The internal control was GAPDH. Data represent the mean ± S.E. of three individual experiments with triplicates. N.S, p > 0.05, *p < 0.05 and**p < 0.01