Fig. 3

Effect of Aurora-A upregualtion on in vitro radiosensitivity of parental HCC cells. a RT-PCR and Western blot detection of Aurora-A mRNA and protein expression in the stably transfected HCC cells (HepG2/Auro or HepG2/control). The internal control was GAPDH. b A representative image of colony formation in the stably transfected HCC cells treated with various dose of IR (0.0 and 4.0 Gy) after 14 days. c Survival fractions of the stably transfected HCC cells were obtained from the results of the MTT assays. d Flow cytometric detection of apoptosis in the stably transfected HCC cells treated with various dose of IR (0.0 and 4.0Gy). Data represent the mean ± S.E. of three individual experiments with triplicates. *p < 0.05 and**p < 0.01