Fig. 2

Effects of Lapatinib on the expression and activity of proteins involved in EGFR/ERBB2 signalling pathways in high and low metastasis NPC cell lines. The high 5-8F and low 6-10B metastatic NPC cells were treated with 5 μM Lapatinib for 0. 4, 8, 24, 48 h. Protein lysates from whole-cell extracts were collected and then analysed by western blotting using the antibodies against ERBB1, P-ERBB1, ERBB2, P-ERBB2, ERBB3, P-ERBB3, AKT, P-AKT, SIRT1, SIRT2, SIRT6, p300, JNK, P-JNK, p38, P-p38, FOXO3, P-FOXO3(T32), FOXM1, and β-tubulin. Molecular markers are shown on the right panel of each blot. Representative results of three repeats are shown. Protein levels were determined by using ImageJ and the protein levels relative to β-tubulin are shown below protein bands as ratios to the 0 h Lapatinib controls