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Fig. 1 | BMC Cancer

Fig. 1

From: CRL4 ubiquitin ligase stimulates Fanconi anemia pathway-induced single-stranded DNA-RPA signaling

Fig. 1

CUL4A/B depletion potentiates ICL cytotoxicity. a HeLa cells were incubated for 48 h with cisplatin (panel on the left) or MMC (panel on the right) together with MLN4924 as indicated (N = 5–10 experiments, error bars show s.e.m.). Cell viability is given as the percentage of controls not exposed to cisplatin. b HeLa cells were transfected with indicated siRNA, incubated with 5 μM cisplatin and tested after 48 h. Viability is expressed as the percentage of control values obtained in the absence of cisplatin (N = 3–5); siNC, non-coding RNA control. Asterisks indicate significantly lower viability in depleted cells relative to non-coding controls (*P < 0.05 and **P < 0.01, unpaired two-tailed t-test). c Cells were transfected with the indicated siRNAs, incubated with cisplatin and tested for viability after 48 h (N = 5). d Cell viability after exposure to MMC (N = 5). e Cytotoxicity assays measuring the release of LDH from siRNA-transfected cells during 48-h treatments with cisplatin (N = 5–10). f Colony-forming assays after exposure of siRNA-transfected cells to the indicated cisplatin concentrations. The resulting colony numbers are normalized to non-exposed controls (N = 5)

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