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Fig. 6 | BMC Cancer

Fig. 6

From: β2AR-HIF-1α-CXCL12 signaling of osteoblasts activated by isoproterenol promotes migration and invasion of prostate cancer cells

Fig. 6

Effects of HIF-1α-CXCL12 signaling in osteoblasts on migration and invasion of prostate cancer cells induced by osteoblasts. a Schematic of the MC3T3 E1/primary osteoblast-PC-3/DU145 co-culture transwell migration and invasion assays. b PC-3/DU145 cells and primary osteoblasts were transfected with siRNAs of CXCR4 and HIF-1α overexpression plasmid, respectively, for 48 h and then expression of CXCR4 (left) and HIF-1α (right) were detected by qRT-PCR and Western blot, respectively (n = 3). c Migration and invasion of PC-3 and DU145 cells were measured over 12 h and 24 h, respectively, in the co-culture with MC3T3E1 osteoblasts with overexpression of HIF-1α or combined with knockdown of CXCR4 in PC-3 and DU145 cells (left). Quantification of relative migration and invasion (right) (n = 3). d Migration and invasion of PC-3 and DU145 cells were measured over 12 h and 24 h, respectively, in the co-culture with primary osteoblasts with overexpression of HIF-1α or combined with knockdown of CXCR4 in PC-3 and DU145 cells (left). Quantification of relative migration and invasion (right) (n = 3). Scales bars, 100 μm; Data represent the mean ± SEM. **p < 0.01, ****p < 0.0001; OE: overexpression

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