Fig. 4

Osteoblasts pretreated with ISO induce EMT of prostate cancer cells. a E-cadherin and Vimentin of EMT markers were measured by Western blotting in PC-3 and DU145 cells treated with or without 10 μM ISO for 24 h (left). Quantitative analysis of relative expression (n = 3) (middle and right). b E-cadherin and Vimentin were measured by Western blotting in PC-3 and DU145 cells co-cultured with MC3T3E1 cells pretreated with or without 10 μM ISO, or 10 μM ISO combined with 50 μM ICI118,551, for 24 h (up). Quantitative analysis of relative expression (n = 3) (bottom). c E-cadherin and Vimentin were measured by Western blotting in PC-3 and DU145 cells co-cultured with primary osteoblasts pretreated with or without ISO, or 10 μM ISO combined with 50 μM ICI118,551 (up). Quantitative analysis of relative expression (n = 3) (bottom). Data represent the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, and n.s. not significant