Fig. 5
From: Role of protein arginine methyltransferase 5 in group 3 (MYC-driven) Medulloblastoma
Therapeutic efficacy of PRMT5 inhibitor EPZ015666 in medulloblastoma cell lines. a MTT assay showing the dose-dependent effects of EPZ015666 (0.1–10 μM) on non-MYC (Daoy, ONS-76) and MYC-amplified (D-283, HD-MB03, D-341) medulloblastoma cell growth. The values represent the means ± SD from four wells of 96-well plates. The percentage of cell viability is relative to control vehicle-treated cells. *, p < 0.05; **, p < 0.01, ***, p < 0.001 (b) Annexin-V assay showing effect of EPZ015666 on apoptosis in HD-MB03 cells. *, p < 0.05; **, p < 0.01, ***, p < 0.001 (relative to ‘0’ (control vehicle). c Cell cycle profile in EPZ015666-treated HD-MB03 cells. d Western blot analysis for the expression of the indicated key proteins in EPZ015666-treated HD-MB03 cells. The values given below each western blot are showing the densitometric quantification of each protein expression relative to the control after β-Actin normalization