Fig. 4

WT1-AS up-regulated p53 and inhibited CSCC cell proliferation. SiHa and C-33A cells were transfected with WT1-AS and p53 expression vectors, followed by the confirmation of over-expression of WT1-AS and p53 by qPCR at 24 h post-transfection (a). In addition, qPCR and western blot analysis showed significantly up-regulated p53 after WT1-AS over-expression at both mRNA and protein levels in both SiHa (left) and C-33A (right) cell line (b). In section (b), for each cell line left panel showed the normalized expression level of p53 mRNA, middle panel showed the representative western blot images, and right panel showed the normalized western blot data. In contrast, qPCR analysis showed that p53 over-expression failed to affect WT1-AS (c). Moreover, p53 and WT1-AS over-expression resulted in inhibited proliferation of CSCC cells (d). All experiments were repeated 3 times and mean values were presented. (*, p < 0.05)