Fig. 2
Estrogen induction of cyclin D1 expression and promoter activity is reduced by over- expression of androgen receptor. a Western blotting analysis of Cyclin D1 (CD1). MCF-7 and MCF-7/AR cells were treated as indicated. Actin was assessed as control of protein loading. b Quantitative Real Time RT–PCR from MCF-7 and MCF-7/AR cells treated as indicated. 18S rRNA was determined as control. Columns are the mean of three independent experiments each in triplicate; bars, SD; *p ≤ 0.05 vs untreated MCF-7 cells; □p ≤ 0.05 vs. E2-treated MCF-7 cells; ●p ≤ 0.05 vs E2 + DHT treated MCF-7 cells. c MCF-7 and MCF-7/AR cells were transiently transfected with pCD1prom-Luc and treated as indicated. Columns are mean of three independent experiments and expressed as fold induction over untreated, which was assumed to be 100%; bars SD; *p ≤ 0.05 vs untreated MCF-7 cells; □p ≤ 0.05 vs. E2-treated MCF-7 cells; ●p ≤ 0.05 vs E2 + DHT treated MCF-7 cells; ▲p ≤ 0.05 vs. E2-treated MCF-7/AR cells; ○p ≤ 0.05 vs. E2 + DHT treated MCF-7/AR cells