Fig. 1

NER process is impaired when DDB2-PCNA interaction is lost. HEK293 stable clones expressing DDB2 wild-type (DDB2^Wt) or mutated protein (DDB2^PCNA-) were transiently transfected with pEFGP-N1 UV-irradiated plasmid and pmRFP-N2 control plasmid. At 16 and 48 h after transfection, the cells were collected and analysed using in vivo cytofluorimetric protocol (see Materials and Methods section). In a GFP and RFP positive cells are reported and the M.F.I. is shown. b Statistical analysis of GFP/RFP ratio from three independent experiments is reported. * p < 0.05