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Fig. 2 | BMC Cancer

Fig. 2

From: Dietary-phytochemical mediated reversion of cancer-specific splicing inhibits Warburg effect in head and neck cancer

Fig. 2

Effect of curcumin treatment on splicing of PKM gene. (a) RPS16 normalized qRT-PCR in curcumin-PEC treated versus control-PEC using the indicated primers. (b) Western blot showing the protein level of PKM1 and PKM2 in curcumin-PEC and control-PEC treated HNC cells, GAPDH act as a loading control. (c) MeDIP in curcumin-PEC versus control-PEC in H157 cells and qRT-PCR relative to input. (d-e) ChIP in H157 cells treated with curcumin-PEC versus control-PEC using (d) BORIS and (e) RNA Pol II antibody, followed by qRT-PCR relative to input and normalized to RPS16. Three independent experiments were conducted with mean values ± SD. P-value calculated using two-tailed Student’s t-test, * P < 0.05, ** P < 0.01, *** P < 0.001, ns = non-significant

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