Fig. 4

SMAD4 Y353C attenuates the inhibitory effect of SMAD4 on cell migration and invasion. The images of SW1990 (a~f) and PANC-1 (g~l) cells are representative of three independent experiments (magnification 40×). The relative migratory area is shown on the right. Migration (m, n) and invasion (o, p) were determined in Transwell assays of SW1990 and PANC-1 cells. The results of the quantification of cell migration and invasion are shown (right). All the statistical data were analysed by one-way ANOVA. All data are shown as the mean ± SD of 3 independent experiments performed in triplicate (*P < 0.05 vs SMAD4 wt or NC)