Fig. 3

HDGF triggered AKT/HIF-1α/NF-κB signaling in SCC4 oral cancer cells. a-d Cells were treated with recombinant HDGF (1–100 ng/ml) for 24 h and then harvested for total protein extraction. The cell lysates were separated by SDS-PAGE and detected by Western blotting with the indicated primary antibodies. β-actin was used as an internal control for loading and transfer. Data were mean of three experiments. *, P < 0.05; **, P < 0.01; ns, not statistically significant