Fig. 2From: Novel HDGF/HIF-1α/VEGF axis in oral cancer impacts disease prognosisEffect of HDGF on VEGF expression in oral cancer cells. SCC4 cells were treated with the indicated concentration of recombinant HDGF protein for 24 h before harvest. a Relative gene expression levels of VEGF were analyzed by SYBR green-based RT-PCR. Data are expressed as the fold change with respect to the control group (means ± SD of triplicate experiments). b Cell lysates were analyzed using Western blotting, and the protein levels of VEGF/β-actin were measured and quantified. c The secreted VEGF protein levels in the supernatants were measured by Western blotting. Ponceau S staining was used as a loading control. d Levels of secreted VEGF protein (pg/ml) were detected by enzyme-linked immunosorbent assay (ELISA) in triplicate experiments. Data were mean of three experiments. *, P < 0.05; **, P < 0.01; ns, not statistically significantBack to article page