Fig. 9From: Suppression of nicotinamide phosphoribosyltransferase expression by miR-154 reduces the viability of breast cancer cells and increases their susceptibility to doxorubicinLuciferase reporter assay verifying the predicted interaction between miR-154 and 3′-UTR of NAMPT. PsiCHECK2 vector harboring NAMPT 3′-UTR or the mutant form of miR-154 recognition element (NAMPT-MRE-tandem-mut) were co-transfected with microRNA-related oligonucleotides into HEK293T. Firefly luciferase activity was normalized with respect to Renilla luciferase as control. The experiments were repeated at least three times. * P < 0.05, ** P < 0.01Back to article page