Fig. 6

MDA-MB-231 transendothelial migration and VEGF-A production. a) Transendothelial cell migration assay showing the effect of endothelial cell irradiation (4 Gy) in the absence or presence of ADAM10/17 inhibitors on the transmigration of MDA-MB-231 breast tumor cells (n ≥ 3). b) VEGF-A content in MDA-MB-231 cell culture supernatants measured by ELISA 24 h after irradiation (mock or 4 Gy; n ≥ 3). c and d) Immunoblot analysis of VE-cadherin expression after irradiation (4 Gy), after treatment with recombinant VEGF-A (100 ng/ml), and after treatment with conditioned medium (CM; harvested after 24 h) from non-irradiated or irradiated (4 Gy) MDA-MB-231 cells (C; n = 2) and U-373 MG cells (D; n = 3) (lysates prepared after 24 h or 2 h in case of VEGF-A treatment). Data are absolute values (b) or relative to those of controls (a, c) and shown as means ± standard deviations. Statistics: t-test, *p < 0.05, **p < 0.01