Fig. 4
From: Overexpression of iASPP is required for autophagy in response to oxidative stress in choriocarcinoma
Effect of iASPP on autophagy. a Higher expression of LC3 was demonstrated in HM than normal placentas by immunohistochemistry (normal vs HM *; P = 0.043). Scale bar, 100 μm. b Choriocarcinoma cell lines, BeWo (2), JEG-3 (3) and JAR (4), expressed more LC3-II than normal trophoblast cell line HTR8/SVneo (1). The LC3 bands were quantified by using ImageJ. The LC3-II to LC3-I ratio on each cell line was analyzed and listed. c Knockdown of iASPP in choriocarcinoma cells decreased Atg5 and LC3-II expressions. d More GFP-LC3 puncta (arrows) were observed in choriocarcinoma cells treated with scramble control than those cells with iASPP knockdown. All the cells were treated with Bafilomycin A1 (20 nM) for 6 h before captured under microscope (200X)