Fig. 2
From: Overexpression of iASPP is required for autophagy in response to oxidative stress in choriocarcinoma
Knockdown of iASPP affected the growth of choriocarcinoma cells. a Choriocarcinoma cells with iASPP knockdown by using two siRNA (siiaspp#1 & siiaspp#2) grew slower than those transfected with scramble control (si) as indicated in MTT assay. (For JEG-3, si vs siiaspp#1, P = 0.001; si vs siiaspp#2, P = 0.002. For JAR, both si vs siiaspp, P < 0.001). b Both JEG-3 and JAR cells with iASPP silencing formed less colonies than the scramble control. (For JEG-3, si vs siiaspp#1, P = 0.03; si vs siiaspp#2, P = 0.19]; For JAR, si vs siiaspp#1, P = 0.007, si vs siiaspp#2, P = 0.01). c The incorporation of BrdU was less in choriocarcinoma cells with iASPP knockdown than the scramble control. (For JEG-3, si vs siiaspp#1, P = 0.03; si vs siiaspp#2, P = 0.008; For JAR, si vs siiaspp#1, P = 0.01; si vs siiaspp#2, P = 0.004 (d) No apparent increase in cleaved caspase 3 (cl. casp3) was seen after iASPP was downregulated. e Trypan blue exclusion assay was used to assess the number of viable cells (left panel) and dead cells (right panel) under different transfection conditions. f TUNEL assay was used to measure the presence of DNA breaks. Cisplatin (CDDP, 10 μM for 24 h) treated cells were used as positive controls. g Histograms showing different cell cycle phases of choriocarcinoma cells with or without iASPP knockdown