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Fig. 3 | BMC Cancer

Fig. 3

From: Autophagy inhibition enhances Matrine derivative MASM induced apoptosis in cancer cells via a mechanism involving reactive oxygen species-mediated PI3K/Akt/mTOR and Erk/p38 signaling

Fig. 3

MASM increased autophagic flux in MDA-MB-231. a MDA-MB-231 were treated with MASM (60 μg/ml) with or without CQ (10 μM), and the immunofluorescent was adopted to detect the LC3 puncta within the cells; b MDA-MB-231 were treated with MASM (60 μg/ml) with or without CQ, and western blot was adopted to detect the expression level of LC3B-II; c Quantitative analysis of bands intensities of LC3B-II by Image J. Data are representative of three independent experiments. *P < 0.05, **P < 0.01

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