Fig. 4

The effect of GX 15–070 used alone in NB cancer stem cells viability (a) and expression of Bcl-2 in NB CSCs (b). Cells viability of CD133 sorted SK-N-DZ cells treated for 48 h with GX 15–070 were measured using the standard MTT (The statistical difference between CD133^high and CD133^low was calculated with Fisher’s exact test) (a). Immunoblotting analyses were performed on protein lysates from SK-N-DZ cells. Anti-CD133 and Bcl-2 antibodies were used (b)