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Fig. 2 | BMC Cancer

Fig. 2

From: GX15–070 (Obatoclax), a Bcl-2 family proteins inhibitor engenders apoptosis and pro-survival autophagy and increases Chemosensitivity in neuroblastoma

Fig. 2

GX 15–070 induced cell death via apoptosis pathway. a Western blotting demonstrated Bcl-2 and cleaved caspase-3 expressions in SK-N-DZ and IGR-NB8 cells treated with GX 15–070. b SK-N-DZ and IGR-NB8 cells treated with different concentrations of GX 15–070 ranging from 0.078 to 5 μM for 48 h. Induction of apoptosis detected by flow cytometric analysis using Annexin V-FITC and 7-AAD staining. c 5X103 SK-N-DZ and IGR-NB8 cells treated with different concentrations of GX 15–070 ranging from 0.05 μM to 5 μM in combination with Z-VAD-FMZ 20 μM for 48 h. Cell viability determined by MTT assays. Viability was calculated as the percentage of living cells in treated cultures compared to those in control cultures. Each value represents the mean ± SEM (n = 3)

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