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Fig. 6 | BMC Cancer

Fig. 6

From: BC200 overexpression contributes to luminal and triple negative breast cancer pathogenesis

Fig. 6

Effect of BC200 on (a) migration and (b) invasion. MDA-MB-231 cells overexpressing BC200 were subjected to real time cell analysis migration (upper left) and invasion (lower left). Left panels (A and B) show the real time results of cells being recorded every 15 min for 24 h. Right panels (A and B) show results at end point (24 h after seeding 20,000 cells on wells for migration – or wells coated with matrigel for invasion). Results are representative of 3 independent cell infections (n = 3) with average of 4 replicates in each independent experiment. LncE = lncEPCAM = BC200; Neg ctrol = negative control – no serum added to the lower chamber of the RTCA plates. For the invasion experiment, twenty thousand (20,000) cells/well were seeded on matrigel coated wells and were let to invade through the upper chamber to the lower chamber for 24 h

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