Fig. 3

Carmine staining of mammary gland whole mount tissue. Whole mount carmine alum staining of ductal epithelium reveals the presence of AB1 (1), AB2 (2) and lobules (3) in respective groups [a-control (normal saline, 3 ml/kg, p.o.); b-toxic control (MNU 47 mg/kg, i.v.); c (MNU + Tadalafil; 47 mg/kg i.v. + 2 mg/kg p.o.) and d (MNU + Tadalafil; 47 mg/kg i.v. + 4 mg/kg p.o.)]. The extent of AB and lobules formation is excessive in the toxic group (b) which was subsided after Tadalafil treatment (c & d). The extent of AB and lobules formation is excessive in the toxic group (b) which has been subsided with respective treatment groups (c & d). The ABs score, lobules and DF scores were also higher in toxic control which was subsided after Tadalafil treatment in a dose dependent manner