Fig. 6

miR-101-3p/BIRC5 axis regulates MM cell viability in BTZ context. a,b RPMI-8226 cells were transfected transiently with cDNA ORF clone of human BIRC5 or the empty vector using lipofectamine 3000. Cells were washed 72 h after transfection, incubated with 5 nM BTZ or CFZ for 48 h and viability was examined in MTT assay (b). The same cells were also transfected in parallel with synthetic inhibitor of miR-101-3p or the scrambled control. Cell lysate was prepared 48 h after transfection and applied to WB for measuring survivin protein level. For these experiments, signals from the WB bands were captured using the ChemiDoc XRS+ System (Bio-Rad). For densitometric quantification of WB bands, we used ImageJ software. The densities of survivin bands were divided by densities of their relevant actin bands and reported as shown