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Fig. 5 | BMC Cancer

Fig. 5

From: Ectopic expression of BIRC5-targeting miR-101-3p overcomes bone marrow stroma-mediated drug resistance in multiple myeloma cells

Fig. 5

miR-101-3p overexpression or BIRC5 silencing triggers apoptosis of MM cells and partially overcomes stroma-induced BTZ resistance. a miR-101-3p or miR-NC transduced RPMI-8226 and MM.1S cells were treated with 5 nM BTZ for 24 h and percent apoptosis was determined using APC-annexin V/PI staining in flow cytometry. Figures are representatives of two separate experiments. b MM cells were transfected with BIRC5 siRNA using Lipofectamine RNAiMAX or scrambled control. Twenty four hours post-transfection, samples were treated with 5 nM BTZ in the presence or absence of HS-5 cells and incubation extended to another 24 h. Cells were then harvested for annexin-V/PI apoptosis analysis in flow cytometry and immunoblot detection of survivin protein. Graphs show the data analysis from two separate experiments, *p < 0.05, **p < 0.01, ***p < 0.001

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