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Fig. 2 | BMC Cancer

Fig. 2

From: Ectopic expression of BIRC5-targeting miR-101-3p overcomes bone marrow stroma-mediated drug resistance in multiple myeloma cells

Fig. 2

BIRC5 mRNA or survivin protein is upregulated by BMSCs in MM cells mainly through direct cell-cell adhesion even in the presence of BTZ. a MM cells were cultured for 24 h in the following settings: cultured alone with or without 5 nM BTZ, co-cultured with HS-5 cells with or without 5 nM BTZ, co-cultured with HS-5 cells pre-treated with BFA or separated with a TW insert. Cells from all conditions were harvested, cDNAs isolated and used in real time PCR for analysis of BIRC5 mRNA expression. Analyzed data are from three separate experiments, *p < 0.05, **p < 0.01, ***p < 0.001. b Primary malignant plasma cells from two MM patients were treated and analyzed as in A but in a 12 h incubation period. c MM cells were co-cultured with HS-5 cells or patient-derived BMSCs for 24 h in the absence of BTZ and survivin protein was measured using WB. MM cells cultured alone were used as controls. d RPMI-8226 cells were cultured for 24 h in the following settings: cultured alone with or without 5 nM BTZ, co-cultured with HS-5 cells with or without 5 nM BTZ, co-cultured with HS-5 cells pre-treated with BFA or separated with a TW insert. Cells from all conditions were harvested and applied to WB for measuring survivin protein. e The same scenario as explained in D was applied to RPMI-8226 and MM.1S cells but in the context of MM patient-derived BMSCs (SC). For densitometric quantification of WB bands, we used ImageJ software. The densities of survivin bands were divided by densities of their relevant actin bands and reported as shown

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