Fig. 4

In vivo the capability of inhibiting tumor growth of the T-lymphocytes treated with IFN-γ or/and 0.1 μg/ml nivolumab. a Schematic diagram for the dosing regimen of T-lymphocytes treated with IFN-γ or/and 0.1 μg/ml nivolumab in subcutaneous tumor-bearing mice. b The capability of inhibiting tumor growth in each group throughout the treatment period. c Cross-comparison between mice treated with Ctrl-T, IFN-γ-T, 0.1 μg/ml nivolumab-T, or 0.1 μg/ml nivolumab+IFN-γ-T. d Measurement of subcutaneous tumor size at 31 days after inoculation. A significant difference was obtained between 0.1 μg/ml nivolumab+IFN-γ-T and 0.1 μg/ml nivolumab, IFN-γ-T, or Ctrl-T. Data shown are mean ± standard deviation, two-tailed t test, *p < 0.05, **p < 0.01. Four biological replicates and three technical replicates were made in each group. e Immunohistochemistry of CD8+ T cells in tumor sections from mice treated with Ctrl-T, IFN-γ-T, 0.1 μg/ml nivolumab-T, or 0.1 μg/ml nivolumab+IFN-γ-T (scale bar = 100 μm). Red fluorescence refers to CD8+ T cells. The number of CD8 + T cells in each microscopic field were counted for analysis. Data shown are mean ± standard deviation, two-tailed t test, **p < 0.01, ***p < 0.001, ****p < 0.0001. Four biological replicates and two immunofluorescence sections of each biological replicate were used for statistics (n = 8)