Fig. 2

PD-1 expressions on T-lymphocytes treated with IFN-γ or/and nivolumab. a PD-1-blockade effect was analysed though the measurement of PD-1 expression, which is detected by flow cytometry. Compared with hIgG4 (green block), PD-1 expression was decreased along with the increase of nivolumab concentration (red point). Pembrolizumab was applied as a positive control. b The PD-1 expressions on CD8+ T-lymphocytes with 7 days’ treatment were measured using flow cytometry. Flow cytometry pseudo colour of lymphocytes and CD3 + CD8+ cells and representative smoothing pseudo colour of PD-1+ cells are displayed. c The level of PD-1 expressions was exhibited in the control group (black box), IFN-γ alone group (green box), 0.1 μg/ml nivolumab alone group (blue box), 10 μg/ml nivolumab alone group (blue stripes), combination treatment of IFN-γ and 0.1 μg/ml nivolumab group (red box), and 10 μg/ml mAb + IFN-γ group (red stripes). Data shown are mean ± standard deviation, two-tailed t test, *p < 0.05, ***p < 0.001, ****P < 0.0001, NS p > 0.05. Four biological replicates and three technical replicates were made in each group