Fig. 6

Sequential targeting of different antigens increases CAR-T cell efficacy in vitro. a Scheme of the experimental setting depicting treatment option I and II. b CD171 and GD2 cell surface expression on WERI-Rb1 cell line is shown on day 3 and 6 after treatment. Retinoblastoma cells were initially treated on day 0 and sequential treatment was conducted on day 3 at a 1:5 E:T ratio, respectively (mean ± SD, n = 3). C. For cell line RB355, CD171 and GD2 expression is illustrated as in b (n = 3). d Lysis of WERI-RB1 and RB335 is shown after initial treatment with CD171- or GD2-specific CAR-T cells and on day 3 and after sequential treatment on day 6 (1:5 E:T ratio, n = 3). Mock-transduced T cells serve as negative control. Treatment is specified in the table below. *, p ≤ 0.5; **, p ≤ 0.01