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Fig. 5 | BMC Cancer

Fig. 5

From: A way to understand idiopathic senescence and apoptosis in primary glioblastoma cells – possible approaches to circumvent these phenomena

Fig. 5

Co-injecting glioblastoma tumor cells with basement membrane matrix proteins enables their growth in vivo. a Schematic workflow of sequential in vitro/in vivo culturing of glioblastoma cells. Primary GB tumors from three patients (GB6, GB8 and GB9) were maintained for total ± 30 weeks in vivo vs ± 8 weeks in vitro, before they lost original mutational profile. P – passage, T – propagation in vivo; b Two separate groups were injected with <104 cells/mouse from fresh GB9 spheres, either suspended in PBS or Matrigel; c Strong GFAP (+) cells from early GB9 sphere were injected subcutaneously into mice; d-e BrdU (+) GB9 cells with decreased GFAP expression and altered morphology removed from mice after 3rd transfer; f-g EGFRvIII and EGFRWT expression in GB9 cells from mice transfers and further in vitro propagations; h-i EGFRvIII (characterized by deletion of exons 2–7) copy number gain in passage 0 and early transfer in mice is gradually lost both, in further passages of in vitro culture or mice transfers. For MLPA results error bars indicate SD, while for Real-time PCR results SEM. Statistical significance calculated by two-way ANOVA analysis with post-analysis Bonferroni’s multiple comparisons test. *, p < 0.05; **, p < 0.01; ***, p < 0.005

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